NOAA Great Lakes Environmental Research Laboratory

The latest news and information about NOAA research in and around the Great Lakes


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Millions of Microbes: The Unexpected Inhabitants of Lake Huron’s Underwater Sinkholes

When most people think of sinkholes, a massive cavity in the ground opening up and swallowing a car is what usually comes to mind. But when scientists at the NOAA Great Lakes Environmental Research Laboratory (GLERL) hear “sinkholes,” their minds jump to an unusual place — the bottom of a Great Lake.

Aerial view of research boat on green water
Researchers on GLERL’s R/V Storm study sinkholes in northern Lake Huron off the coast of Alpena, Michigan. (Credit: David J Ruck/Great Lakes Outreach Media)

Thousands of years ago, off the coast of Alpena, Michigan, patches of ground beneath Lake Huron collapsed to form a series of underwater sinkholes — some measuring hundreds of feet across and up to 60 feet deep. You may have read this NOAA.gov article about how these sinkholes are contributing water to Lake Huron, but did you know they also support a huge kingdom of microorganisms?

Microbes might be tiny, but they’re one of the biggest research topics in the Great Lakes. They thrive near the sinkholes because the groundwater seeping in has the perfect chemistry for their survival: low oxygen levels and lots of chloride and sulfate, which come from the dissolved limestone underlying the lake. These factors make the sinkholes inhospitable for fish and other wildlife normally found in the Great Lakes, which means these microbes have a much easier time surviving there than other creatures. With perfect living conditions and little competition, they’re so abundant that they form purple, green, and white microbial mats that cover the lake floor like a colorful carpet.

Floor of Lake Huron covered by purple and white microbial mats with bubbles in them.
Purple microbial mats in the Middle Island Sinkhole in Lake Huron, June 2019. Small hills and “fingers” like this one in the mats are caused by gases like methane and hydrogen sulfide bubbling up beneath them. (Credit: Phil Hartmeyer, NOAA Thunder Bay National Marine Sanctuary)

Scientists at GLERL are collaborating with partners from the University of Michigan and Grand Valley State University to see just what these microscopic lake dwellers can teach us. This video by Great Lakes Outreach Media highlights how they can even give us a deeper insight into the history of Earth itself.

Associate Professor Greg Dick from the University of Michigan discusses cyanobacteria’s important role in Earth science. This clip is from Great Lakes Outreach Media’s upcoming documentary, “The Erie Situation.”

Some sinkholes are so deep that sunlight can’t reach them, but that doesn’t stop some microbes from calling them home. They’re able to live their entire lives in complete darkness, because they get their energy from the added minerals in the water rather than from sunlight — a process called chemosynthesis. But whether they need sunlight or not, several of the microbial species present have proven to be full of surprises.

“In the near-shore systems, the cyanobacteria we found have DNA signatures that come closest to comparing to the cyanobacteria found at the bottom of a lake in Antarctica. So that’s a strange coincidence,” said Steve Ruberg, the scientist in charge of sinkhole research at GLERL. “Some of the other bacteria we’ve found in the deeper systems have only been found off the coast of Africa.”

Fish sitting on a rock, which is covered by purple and white microbes
A burbot resting on rocks covered in purple and white microbial mats inside the Middle Island sinkhole in Lake Huron. (Credit: Phil Hartmeyer, NOAA Thunder Bay National Marine Sanctuary)

The particular sinkholes we’re studying are located within NOAA’s Thunder Bay National Marine Sanctuary, an area of Lake Huron that’s federally protected for the purpose of preserving nearly 200 shipwrecks. In fact, the only reason we know about these sinkholes is because they were discovered by accident only 18 years ago, on a research cruise documenting the shipwrecks.

Close up of rocks covered in  purple, white and green microbes on the bottom of Lake Huron, with a diver in the background.
A diver observes the purple, white and green microbes covering rocks in Lake Huron’s Middle Island Sinkhole (Credit: Phil Hartmeyer, NOAA Thunder Bay National Marine Sanctuary)

So why did this microbial paradise come into existence in the first place? The story goes back much further than the sinkholes’ discovery in 2001. About 400 million years ago, before the Great Lakes even existed, a layer of limestone bedrock formed beneath what is now Lake Huron. Then around 10,000 years ago, underground caves were created when a chemical reaction between the limestone and acidic groundwater dissolved away holes in the bedrock. All that was left were weakly supported “ceilings” that eventually collapsed into the sinkholes we — and the microbes — know and love today.

Close up of rocks covered in purple, white and green microbes on the floor of Lake Huron
Purple cyanobacteria and white chemosynthetic mats on the floor of Lake Huron with Lowell Instruments current meter. (Credit: Phil Hartmeyer, NOAA Thunder Bay National Marine Sanctuary)

Since Lakes Michigan and Erie have the same limestone bedrock as Lake Huron, GLERL scientists think these lakes could be home to more of these fascinating underwater features. So while the excitement of this fieldwork has died down for the year, our research on Great Lakes sinkholes and their tiny inhabitants is far from over.


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Exploring the diversity of native species with Great Lakes Water Life

From prehistoric-looking lake sturgeon to colorful crayfish, the Great Lakes are alive with thousands of remarkable native species. To document and celebrate the diversity of fauna native to the Great Lakes, NOAA-GLERL has partnered with US EPA and the Great Lakes Sea Grant Network to launch the new Great Lakes Water Life database: a comprehensive, accessible inventory of aquatic species found throughout the region.

Three researchers aboard a boat hold up a large lake sturgeon that is as long as they are tall.
Researchers hold a lake sturgeon, one of the many species native to the Great Lakes (photo courtesy of Todd Marsee, Michigan Sea Grant).

Great Lakes Water Life (GLWL) is designed to support environmental researchers and managers by hosting a broad range of ecological information and tools: identification guides for native species, records of rare or unfamiliar taxa, lists of expected species in a specific area, summaries of broad-scale biodiversity patterns, and more. This site is also available for public use to students, citizen scientists, and other Great Lakes residents who want to learn about native species in their area, providing new opportunities for outreach and education online.

“This user-friendly database captures the unique biological diversity of the Laurentian Great Lakes,” said Debbie Lee, Director of the NOAA Great Lakes Environmental Research Laboratory.  “The search function invites the curious to learn about the amazing water life native to the largest surface freshwater system on earth.”

A screenshot of the Great Lakes Water Life home page, featuring an about section, a search tool, additional resources, and a contribution portal.
The new Great Lakes Water Life landing page.

GLWL allows users to search for species by taxa, origin, domain, and broad geographic location. Each species result links to taxonomic information, a bibliography of references and sighting information, links into Barcode of Life DNA markers, and more. The database also includes links to other taxonomic keys and field guides to native species, information about the purpose and history of this project, and a user contribution portal where researchers can share new photos, sightings, and collection records to be added to the site.

A screenshot of the Great Lakes Water Life search results, showing several species of native fish.
Users can search for native species to learn more about taxonomic information, geographic location, DNA markers, and more.

This database builds on a previous project known as the “Great Lakes Waterlife Gallery,” originally created in 2002 in support of Sea Grant’s Great Lakes Fisheries Leadership Institute in partnership by NOAA-GLERL and the Great Lakes Sea Grant Network. 

Another NOAA-led regional database, the Great Lakes Nonindigenous Species Information System (GLANSIS), runs in parallel with GLWL to more comprehensively document the non-native aquatic species that have been introduced to the Great Lakes. Cross-linking the two systems helps GLANSIS to provide DNA information on non-native species and identify species that may be expanding their ranges, highlighting the value of the native species inventory to monitoring for and understanding the impact of aquatic invaders. Great Lakes invaders shouldn’t get all the press coverage, however — researchers hope that the Great Lakes Water Life database will help fellow scientists make informed management decisions and help the public get to know more about the unique native creatures that inhabit the Great Lakes.

To learn more about the Great Lakes Water Life database or contribute information, please visit the site or contact Rochelle.Sturtevant@noaa.gov.


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Special Two-Day Science Translation Session at IAGLR 2019

This June, fellow researchers from around the world will gather in Brockport, New York, on the shores of the Erie Canal for IAGLR’s 62nd annual Conference on Great Lakes ResearchHosted by The College at Brockport, State University of New York, the conference will feature four days of scientific sessions and speakers focusing on the theme Large Lakes Research: Connecting People and Ideas. Mark your calendars for June 10-14, 2019. You won’t want to miss it! 

During the conference there will be a special two-day session that highlights the importance of science translation.  The session, Beyond Peer Review: Why You Must Connect Your Science to Stakeholders (and how to do it), will consist of several components—17 formal presentations, a moderated panel discussion, a synthesis discussion with Q&A, as well as a Skills Café. Conference attendees are welcome to join us for any and all portions of this session. We hope to see you there!


Day 1 – Tuesday, June 11th

On Tuesday, the SciComm session will include 12 presentations and a panel moderated discussion with science communication thought leaders Peter Annin (Author), Andrea Densham (Shedd), Sandra Svoboda (DPTV) & TJ Pignataro (Buffalo News). The panelists will explore what they see happening now and what they think the future looks like for connecting people and ideas for large lakes research.

Tuesday Morning (Edwards Hall Room 103)

Tuesday Afternoon (Edwards Hall Room 103)

  • 1:40-3:20 pm – 5 presentations
  • 3:20-3:40 pm – Break
  • 3:40-5:20 pm – 4 presentations

Day 2 – Wednesday,  June 12th

On Wednesday, the SciComm session will continue with 5 formal presentations, and a synthesis discussion in the morning and a Skills Café in the afternoon.

Wednesday Morning (Edwards Hall Room 103)

  • 8:00-9:20 am – 3 presentations
  • 9:20-9:40 am – Break
  • 9:40-10:20 am – 2 presentations
  • 10:20-11:00 am – Interactive Synthesis Discussion and Question & Answer Session with Peter Annin (Author), Andrea Densham (Shedd), Sandra Svoboda (DPTV) & TJ Pignataro (Buffalo News)

Wednesday Afternoon (Edwards Hall Room 102)

  • 1:40 – 5:00 pm – Skills Café  –  This series of short interactive workshops will allow participants to practice a variety of skills that will make them more effective at communicating the “so what” of their research to lay – but key – audiences. 

The Panel: Hear the latest from science communication thought leaders!

Peter Annin, Author and Director of the Mary Griggs Burke Center for Freshwater Innovation

Peter Annin is the director of the Mary Griggs Burke Center for Freshwater Innovation and the author of The Great Lakes Water Wars, the definitive work on the Great Lakes water diversion controversy. Before coming to Northland College in 2015, Peter served as a reporter at Newsweek, the associate director of the Institute for Journalism and Natural Resources, and the managing director of the University of Notre Dame’s Environmental Change Initiative. He continues to report on the Great Lakes water diversion issue and has published a second edition of The Great Lakes Water Wars. 

Andrea Densham, Senior Director of Conservation and Advocacy at the Shedd Aquarium

Andrea Densham joined Shedd Aquarium in 2017 to lead the newly launched Conservation Policy and Advocacy team. Created to enhance Shedd’s position as a policy expert, Densham’s team develops and implements the institution’s policy goals. A government affairs thought leader and advisor, she brings more than 20 years of experience in not-for-profit management, strategic planning, research, and public policy and advocacy.

 

TJ Pignataro, Environmental Reporter for the Buffalo News

T.J. Pignataro has been a staff reporter for The Buffalo News for more than 20 years and the environment and weather reporter since 2013. He holds a juris doctor degree from SUNY Buffalo Law School and is completing his Certificate in Weather Forecasting this spring from the Pennsylvania State University’s Department of Meteorology and Atmospheric Science. TJ uses Twitter to convey Great Lakes environmental news, weather emergencies and Great Lakes science in plain language. 

Sandra Svoboda, Program Director, Great Lakes Now, Detroit Public Television

A nine-month stint with The Associated Press brought Sandra to Detroit … 29 years ago. She earned a bachelor’s in journalism from Indiana University and holds two master’s degrees from Wayne State, one in public administration and one in library and information science. The Special Libraries Association IT Division recognized her research with its 2018 Joe Ann Clifton Student Award for her paper on how Detroit voting dynamics can inform citizen engagement strategies. Sandra has worked for The (Toledo) Blade covering education/children’s issues, Detroit’s Metro Times and FEMA, where she deployed to Louisiana to help coordinate/communicate about community rebuilding/planning efforts for/after disasters. Sandra has won awards for broadcast, print, digital and community engagement work from the Michigan Associated Press, the Michigan Association of Broadcasters, Association of Alternative Newsweeklies, State Bar of Michigan, Michigan Press Association and Society of Professional Journalists-Detroit chapter, and Wayne State’s public administration program recognized her with the Distinguished Alumni Award in 2015 for her work covering Detroit’s bankruptcy. She has taught communications, writing, public policy, and political science at Wayne State University and the University of Michigan-Dearborn. As the Great Lakes region has always been her home Sandra has traveled between Minnesota and Tadoussac, Quebec, both on the water and on land. A competitive sailor, she races hundreds of miles each season on the Great Lakes, and once threw out a pitch at a Detroit Tigers game as recognition of her win with her team at the U.S. Women’s Match Racing Championship. She’s also eaten Asian carp as part of her coverage of invasive species.


The Skills Café: Get help communicating your research!

WHO: Do people’s eyes glaze over when you begin to talk about your research? Do you believe your research has the ability to make a difference, but you’re not sure how to get others excited about it too? Then this session is for you! For the researcher looking to improve their accessibility in attaining broader impacts; the early career professional seeking tips on how to set theirselves apart in a competitive market; the passionate scientist looking for ways to ensure their work makes an impact . . . the Skills Cafe is your opportunity to grow and try new things in a fun and supportive setting.

WHAT: This series of short interactive workshops will allow participants to practice a variety of skills that will make them more effective at communicating the “so what” of their research to lay—but key—audiences. Get tips on interacting with the media, hone your speaking skills, get feedback from a mock interview, and learn from the trials and tribulations of your peers!

WHEN: 1:40-5:00 pm on Wednesday, June 12.

WHERE: Edwards Hall, Room 102

For more information and a detailed schedule of activities stop by the NOAA exhibitor booth.


About IAGLR 2019: The 2019 International Association for Great Lakes Research Conference is hosted by The College at Brockport, State University of New York, June 10-14, 2019. The conference will feature four days of scientific sessions and speakers focusing on the theme “Large Lakes Research: Connecting People and Ideas.”

photo of building in water with skyline of city in backgroun


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NOAA and partners team up to prevent future Great Lakes drinking water crisis

A new video SMART BUOYS: Preventing a Great Lakes Drinking Water Crisis released by Ocean Conservancy describes how NOAA forecast models provide advance warnings to Lake Erie drinking water plant managers to avoid shutdowns due to poor water quality.

An inter-agency team of public and private sector partners, working with the Cleveland Water Department, are addressing drinking water safety for oxygen depleted waters (hypoxia). By leveraging NOAA’s operational National Weather Service and National Ocean Service forecast models and remote sensing for the Great Lakes, NOAA’s latest experimental forecast models developed by its Great Lakes Environmental Research Laboratory can predict when water affected by harmful algal blooms and hypoxia may be in the vicinity of drinking water intake pipes. Advance notice of these conditions allows water managers to change their treatment strategies to ensure the health and safety of drinking water.  

“Hypoxia occurs when a lot of organic material accumulates at the bottom of the lake and decomposes. As it decomposes, it sucks oxygen from the water, can discolor the water and allow for metals to concentrate,” explains Devin Gill, stakeholder engagement specialist for NOAA’s Cooperative Institute for Great Lakes Research, hosted at the University of Michigan.

Low dissolved oxygen on its own is not a problem for water treatment. However, low oxygen is often associated with a high level of manganese and iron in the bottom water that then leads to drinking water color, taste, and odor problems. In addition, the same processes that consume oxygen also lower pH and, if not corrected, could cause corrosion in the distribution system, potentially elevating lead and copper in treated water.

“Periodically, this water with depleted oxygen gets pushed up against the shoreline and the drinking water intakes pipes,” said Craig Stow, senior research scientist for NOAA’s Great Lakes Environmental Research Laboratory. “We have buoys stationed at various places and those guide our models to let us know when conditions are right for upwellings that would move this hypoxic water into the vicinity of the drinking water intakes.”

NOAA provides advanced warning of these events so that drinking water plant managers can effectively change their treatment strategies to address the water quality, which is a huge benefit in the water treatment industry.


For more information on NOAA GLERL’s harmful algal blooms and hypoxia research, visit www.glerl.noaa.gov/res/HABs_and_Hypoxia.

map of great lakes showing colors of model output


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Improving lake effect snow forecasts by making models talk to each other

If you live in the Great Lakes basin and have been on or even near a road recently, you might be feeling unreasonably ragey at the mere mention of lake effect snow. We get it. But bear with us, because we’re doing some cool science we’d like to tell you about. It may even make your commute easier someday, or at least more predictable.

GLERL scientists are working with researchers at the University of Michigan’s Cooperative Institute for Great Lakes Research (CIGLR), the National Weather Service, and NOAA’s Earth Systems Research Laboratory (ESRL) to make lake effect snow forecasts in the Great Lakes better.

NOAA’s high resolution rapid refresh (HRRR) model is the most commonly used weather model for predicting lake effect snow. An experimental version runs on a beastly high-performance computer at ESRL in Colorado, and predicts a whole list of atmospheric variables (including snowfall) every 15 minutes. The model relies on water surface temperature data, collected via satellite, to make its predictions. It’s important to give the model accurate water surface temperatures to estimate evaporation across the Great Lakes, since it is the main driver of lake effect snow.

Unfortunately, satellite temperature data has limitations. If clouds keep satellites from measuring the temperature at a specific location, the weather model will just use the most recent measurement it has. Since it’s especially cloudy in the Great Lakes during the lake effect snow season (late fall and early winter), that data could be days old. Because lake temperatures are changing quite rapidly this time of year, days-old data just doesn’t cut it.

As it turns out, GLERL already has a model that predicts Great Lakes surface temperature pretty well. The Great Lakes Operational Forecast System (GLOFS) spits out lake surface temperatures every hour. If we tell the weather model to use GLOFS output instead of satellite data, it has the potential to do a far better job of forecasting lake effect snow.

Linking two models like this is called “coupling”. GLOFS actually already uses input from HRRR—wind, air temperature, pressure, clouds and humidity data all inform GLOFS’ predictions. We’re just coupling the models in both directions. HRRR will send its output to GLOFS, GLOFS will “talk back” with its own predictions of water surface temperature (and ice cover), and HRRR will produce a (hopefully) more informed prediction of lake effect snow.

Initial results are promising. We used the coupled models to do a ‘hindcast’ (a forecast for the past) to predict lake effect snow for a major event over Lake Erie in November of 2014. They did a significantly better job than without coupling. The figure below shows the difference.

The coupled models improved cumulative snow water equivalent forecasts. Red shows where the model increased snowfall.

You’ll notice a band of blue on the southeastern edge of Lake Erie, indicating that the coupled models predicted less lake effect snow in that area. There’s a band of orange directly to the north of it, where the coupled models predicted more lake effect snow. What you’re seeing is the coupled model predicting the same band of snow, but further north, closer to where it actually fell.

That storm slammed the city of Buffalo, New York, killing 13 people. Better lake effect snow predictions have the potential to save lives.

GLERL and partners will be doing further testing this winter, and if it works out, the model coupling will be carried over in research-to-operations transitions.


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The HAB season is over, but the work goes on

It’s nearly winter here in the Great Lakes—our buoys are in the warehouse, our boats are making their way onto dry land, and folks in the lab are working hard to assess observed data, experiments, and other results from this field season.

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This is a retrospective animation showing the predicted surface chlorophyll concentrations estimated by the Experimental Lake Erie HAB Tracker model during the 2018 season. Surface chlorophyll concentrations are an indicator of the likely presence of HABs. For more information about how the HAB Tracker forecast model is produced and can be interpreted, visit our About the HAB Tracker webpage.

The harmful algal bloom (HAB) season is also long over in the region. The final Lake Erie HAB Bulletin was sent out on Oct. 11, as the Microcystis had declined in satellite imagery and toxins decreased to low detection limits in samples. In the seasonal assessment, sent out by NOAA’s Centers for Coastal Ocean Science on Oct. 26, it was determined that the season saw a relatively mild bloom—despite its early arrival in the lake—and the bloom’s severity was significantly less than that which was predicted earlier in the season. These bulletins and outlooks are compiled using several models. Over the winter, the teams working on the models take what they learn from the previous season, and update their models for future use.

Back in the lab, the HABs team—researchers from both GLERL and the Cooperative Institute for Great Lakes Research (CIGLR)—will spend the winter analyzing data they collected through a variety of observing systems. This summer was packed with the use of new observing technologies, like hyperspectral cameras and the Environmental Sample Processor (in case you missed it, check out this fun photo story of the experimental deployment of a 3rd generation ESP). In addition, GLERL and CIGLR staff maintained a weekly sampling program program, from which scientists are analyzing and archiving samples and conducting experiments.

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Aerial photograph of the harmful algal bloom in Western Basin of Lake Erie on July 2, 2018, (Photo Credit: Aerial Associates Photography, Inc. by Zachary Haslick). Pilots from Aerodata have been flying over Lake Erie this summer to map out the general scope of the algal blooms. In addition to these amazing photos, during the flyovers, additional images are taken by a hyperspectral imager (mounted on the back of the aircraft) to improve our understanding of how to map and detect HABs. The lead researcher for this project is Dr. Andrea VanderWoude, a NOAA contractor and remote sensing specialist with Cherokee Nation Businesses. For more images, check out our album on Flickr.

This lab work is super important for understanding the drivers of toxic algae in the Great Lakes. For instance, in a new study released this month, researchers looking at samples from previous years found that “ . . . the initial buildup of blooms can happen at a much higher rate and over a larger spatial extent than would otherwise be possible, due to the broad presence of viable cells in sediments throughout the lake,” according to the lead author Christine Kitchens, a research technician at CIGLR, who works here in the GLERL lab. This type of new information can be incorporated into the models used to make the annual bloom forecasts.

As you can see, our work doesn’t end when the field season is over.  In spring 2019, when the boats and buoys are back in the water and samples are being drawn from the lakes, researchers will already have a jump on their work, having spent the winter months analyzing previous years, preparing, and applying what they’ve learned to the latest version of the Experimental HAB Tracker, advanced observing technologies, and cutting-edge research on harmful algal blooms in the Great Lakes.


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Photo story: Using an AUV to track algae in Lake Erie

In late July and early September, during the peak of the 2018 harmful algal bloom in the Western Basin of Lake Erie, NOAA GLERL, NOAA National Centers for Coastal Ocean Science (NCCOS), NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) and CIGLR researchers teamed up with a group of scientists and engineers from the Monterey Bay Research Institute (MBARI). Their mission: to test how well a third-generation environmental sample processor (3GESP), mounted inside a long-range autonomous underwater vehicle (LRAUV), can track and analyze toxic algae in the Western Basin of Lake Erie. You can read more about the purpose of this project in this great news story by MBARI’s Kim Fulton-Bennett.

Below is a photo story showing all (well, much) of the hard work that went into this test deployment.

First, the new gear had to be shipped from California to the GLERL laboratory in Ann Arbor, Michigan.

 

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Upon arrival, Jim Birch, Director of the MBARI SURF (Sensors Underwater Research of the Future) Center, & Bill Ussler, MBARI biogeochemist, got straight to work in GLERL’s Marine Instrumentation Lab.

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The inside of the 3G ESP has a lot of moving parts. Since this is the first time the team is testing it in freshwater, before it can go out, everything needs to be fine-tuned to work in a variety of conditions in Lake Erie (more on that later.)

So. Many. Moving. Parts.

 

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Once everything is in working order, the 3GESP gets inserted into an LRAUV or long-range autonomous underwater vehicle (the torpedo-looking thing). This gives the 3GESP the ability to move around in the water all by itself once researchers have set parameters for it. The team has named this particular vehicle, Makai, which is Hawaiian for “toward or by the sea.” Seems appropriate! That’s Brian Kieft, MBARI software engineer, on the right. He plays a crucial role in making sure Makai does her job.

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All hands on deck for a few more tweaks.

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Once everything is installed tightly, helium is added into the canister to check for leaks. CIGLR engineer, Russ Miller, is working with Jim to fill it up.

Now, the team is ready to head out to Lake Erie. Here’s where things start to get exciting!

 

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Before the team sets Makai free to track the algal bloom in the Western Basin of Lake Erie, they must first check her ballast and trim. This is especially important for such a shallow lake (relative to where the team has been testing this technology in the deep canyons of of Monterey Bay off the coast of California.)

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Brian has to do all of the hard work.

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Because, science.

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Time to load Makai onto the NOAA vessel, which is stationed in La Salle, Michigan. Captain Kent Baker, a contractor with NOAA, is in the background operating the crane. Kent takes NOAA and CIGLR researchers and technicians out to bi-weekly sampling stations, helps deploy buoys and other instrumentation, and is at the ready for pretty much anything that needs to happen in Lake Erie.

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Once she’s all settled onto the boat, the team takes Makai to the first deployment location.

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The inaugural deployment was set to match up with the bi-weekly sampling stations.

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Look closely and you’ll see Makai off on her way!

Makai and the team spent nearly two weeks tracking, sampling, adjusting, and learning about using this technology to track algal toxins in Lake Erie.

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The team used the images from GLERL’s Experimental Lake Erie Harmful Algal Bloom (HAB) Tracker to determine where to send Makai.

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Then, they would determine how many samples to take, and program her to go to specific waypoints.

Remember when we said this Lake Erie mission will be different than the ones the team has performed in Monterey Bay? Well, here’s one example of how.

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After a few hours of no communication, and a little hunting, this is how the team found Makai. Two problems here: One, with the propellor up and the nose down, Makai cannot transmit data, including her location, as the transmitter only works above water. And, two, well . . .

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The reason she was nose down in the first place is because Lake Erie is pretty shallow, and she’d taken on quite a bit of mud.

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Once she was all cleaned up, the team set Makai out again to complete the rest of her mission.

Once the deployment was over, the research didn’t stop there.

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Archive samples were taken so that folks back in the lab could further analyze them.

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Here’s GLERL’s Observing Systems and Advanced Technology (OSAT) branch chief, Steve Ruberg (left), along with Paul Den Uyl, a researcher with CIGLR, helping Bill extract the sample filters from the cartridges.

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The filters are being collected for analysis of DNA. The DNA will be extracted from each filter and analyzed. We’re looking at absolute quantity of known microcystin producing toxin genes in samples collected, information on bacterial community composition, and information on eukaryotic organism community composition. The samples will also analyzed through shotgun sequencing. This is where all of the genes in the sample are turned into human readable information and can be combined to make what can be thought of as an organism’s genetic instruction guide (what genes it has). This information will be very helpful in better understanding what causes the algae to be toxic (not all algae is toxic).